Prajesh Shrestha

Research Publications

Peer-reviewed publications in peptide design, drug delivery systems, cancer therapeutics, and autoimmune disease treatment

12
Total Publications
2022-2026
Years Active
5
Research Areas
26+
Total Citations
Book (Editor)
2026
Computational Approaches in Drug Design: Targeting Protein-Protein Interactions
Springer Nature • 2026
2026

Co-edited comprehensive book published by Springer Nature covering computational methodologies for drug design targeting protein-protein interactions. This book provides an in-depth exploration of modern computational tools, molecular modeling techniques, and structure-based drug design approaches for developing therapeutics that modulate protein-protein interactions in various disease contexts.

Computational Drug Design
Protein-Protein Interactions
Molecular Modeling
Structure-Based Design
Book Chapter
2026
Peptides and Peptidomimetics as Inhibitors of Protein-Protein Interactions
In: Computational Approaches in Drug Design: Targeting Protein-Protein Interactions • Springer Nature • 2026
2026

Authored book chapter providing comprehensive coverage of peptide and peptidomimetic design strategies for inhibiting protein-protein interactions. The chapter discusses rational design approaches, structural modifications for enhanced stability and bioavailability, and case studies of successful peptidomimetic inhibitors in therapeutic development.

Peptide Design
Peptidomimetics
PPI Inhibitors
Drug Discovery
Book Chapter
2026
Protein Trimerization and Viral Fusion: Structural Insights and Therapeutic Applications
In: Computational Approaches in Drug Design: Targeting Protein-Protein Interactions • Springer Nature • 2026
2026

Co-authored book chapter exploring the structural biology of protein trimerization in viral fusion mechanisms and their therapeutic implications. The chapter provides insights into how trimeric protein complexes facilitate viral entry and discusses strategies for developing antiviral therapeutics targeting these critical protein-protein interactions.

Protein Trimerization
Viral Fusion
Structural Biology
Antiviral Therapeutics
New Publication
Published 2026
Formulation and Evaluation of Metformin Hydrochloride Sustained-Release and Empagliflozin Immediate-Release Bilayer Tablets
International Journal of Applied Pharmaceutics • Volume 18, Issue 1 • January 2026
2026

This study presents the development and optimization of bilayer tablets containing sustained-release Metformin hydrochloride and immediate-release Empagliflozin for Type 2 diabetes management. Using response surface methodology (RSM) through central composite design (CCD), the formulation was optimized with HPMC K100M and Carbopol 971P polymers. The optimized formulation demonstrated excellent drug release kinetics following the Korsmeyer-Peppas model, indicating anomalous transport driven by both diffusion and polymer relaxation mechanisms. This work demonstrates the integration of experimental design and mechanistic modeling for bilayer tablet formulations, offering a promising approach for improving glycemic control and patient compliance.

Type 2 Diabetes
Bilayer Tablets
Sustained Release
Metformin HCl
Empagliflozin
Response Surface Methodology
An Orally Available Grafted Peptide Targeting Epidermal Growth Factor Receptor Dimers Reduces Non-Small Cell Lung Cancer Tumors in Mouse Models
ACS Pharmacology & Translational Science • Published January 2025
2025

This groundbreaking research presents the development of orally bioavailable grafted peptides that specifically target EGFR receptor dimers, demonstrating significant tumor reduction in non-small cell lung cancer mouse models. The study represents a major advancement in peptide-based cancer therapeutics by successfully combining computational design with rigorous in vivo validation to create clinically viable oral peptide drugs. The grafted peptide design overcomes traditional limitations of peptide therapeutics, including poor oral bioavailability and proteolytic degradation, while maintaining high specificity for EGFR dimers. This work provides a promising new therapeutic approach for NSCLC treatment and establishes a framework for developing oral peptide-based cancer therapies.

Oral Peptide Delivery
EGFR Targeting
NSCLC Therapy
In Vivo Validation
Grafted Peptides
Cancer Therapeutics
Dual upregulation of miRNA-143 and miRNA-506 in non-small cell lung cancer inhibits proliferation, motility, migration, and tumor growth
Frontiers in Drug Discovery • Volume 5 • June 2025
2025

Original research investigating the therapeutic potential of dual miRNA upregulation in non-small cell lung cancer (NSCLC). This study demonstrates that combined upregulation of miRNA-143 and miRNA-506 significantly inhibits cancer cell proliferation, motility, migration, and tumor growth. The research provides insights into miRNA-based therapeutic strategies for lung cancer treatment, highlighting the advantages of combinatorial miRNA approaches over individual miRNA deregulations in regulating tumor progression and metabolic reprogramming.

miRNA Therapy
Non-Small Cell Lung Cancer
Cancer Metabolic Reprogramming
Tumor Growth Inhibition
Molecular Chimera in Cancer Drug Discovery: Beyond Antibody Therapy, Designing Grafted Stable Peptides Targeting Cancer
International Journal of Peptide Research and Therapeutics • February 2025
2025

Developed novel molecular chimeric peptides that combine the specificity of antibodies with the stability and cell-penetrating properties of engineered peptides. These grafted stable peptides demonstrate enhanced tumor targeting, improved pharmacokinetics, and superior therapeutic efficacy compared to traditional antibody-based therapies in cancer treatment.

Molecular Chimeras
Grafted Peptides
Cancer Targeting
Peptide Stability
The Synthesis of BODIPY-TKI Conjugates and Investigation of Their Ability to Target the Epidermal Growth Factor Receptor
Targets • August 2023
2023

Developed novel BODIPY-tyrosine kinase inhibitor (TKI) conjugates designed to specifically target the epidermal growth factor receptor (EGFR). This research combines fluorescent BODIPY dyes with TKI pharmacophores to create dual-functional molecules that enable both therapeutic targeting and real-time imaging of EGFR-expressing cancer cells, providing a powerful tool for theranostic applications in oncology.

BODIPY Conjugates
Tyrosine Kinase Inhibitors
EGFR Targeting
Theranostics
Conformationally constrained cyclic grafted peptidomimetics targeting protein–protein interactions
Peptide Science • July 2023
2023

Developed conformationally constrained cyclic grafted peptidomimetics based on sunflower trypsin inhibitor-1 (SFTI-1) structure for targeting protein-protein interactions. Incorporated dibenzofuran moiety to lock peptides into major conformations, with detailed NMR and surface plasmon resonance studies demonstrating specific binding to CD58 protein. This research provides a framework for designing stable therapeutic peptides through strategic conformational constraints.

Cyclic Peptides
SFTI-1 Grafting
Conformational Constraints
Protein-Protein Interactions
Lyophilized liposomal formulation of a peptidomimetic-Dox conjugate for HER2 positive breast and lung cancer
International Journal of Pharmaceutics • April 2023
2023

Developed an innovative lyophilized liposomal drug delivery system for a peptidomimetic-doxorubicin (Dox) conjugate specifically targeting HER2-positive breast and lung cancers. This advanced formulation enhances drug stability, improves targeted delivery to cancer cells, and reduces systemic toxicity. The lyophilization process ensures long-term storage stability while maintaining the therapeutic efficacy of the peptidomimetic-drug conjugate for precision cancer therapy.

Liposomal Delivery
HER2 Targeting
Peptidomimetic-Drug Conjugates
Cancer Nanomedicine
Assessment of Antitumor and Antiproliferative Efficacy and Detection of Protein-Protein Interactions in Cancer Cells from 3D Tumor Spheroids
Current Protocols • October 2022
2022

Developed comprehensive protocols for evaluating antitumor and antiproliferative efficacy using 3D tumor spheroid models. This methodological framework enables standardized assessment of protein-protein interactions in cancer cells within physiologically relevant 3D microenvironments. The protocol provides detailed procedures for spheroid formation, drug treatment, efficacy evaluation, and protein interaction detection, serving as a valuable resource for cancer research laboratories worldwide.

3D Tumor Spheroids
Cancer Cell Models
Protein-Protein Interactions
Methodological Protocol
Targeting protein–protein interaction for immunomodulation: A sunflower trypsin inhibitor analog peptidomimetic suppresses RA progression in CIA model
Journal of Pharmacological Sciences • April 2022
2022

Developed a novel sunflower trypsin inhibitor (SFTI-1) analog peptidomimetic for treating rheumatoid arthritis through targeted protein-protein interaction modulation. Using a collagen-induced arthritis (CIA) mouse model, demonstrated significant suppression of RA progression through immunomodulatory mechanisms. This research provides a promising therapeutic approach for autoimmune diseases by targeting specific molecular interactions involved in inflammatory responses and joint destruction.

Rheumatoid Arthritis
SFTI-1 Peptidomimetics
Immunomodulation
CIA Model

Research Impact & Themes

Cancer Therapeutics

Advanced peptide-based approaches for cancer treatment including molecular chimeras, drug delivery systems, and EGFR targeting strategies.

7 Publications
Computational Drug Design

Book and book chapters on computational approaches targeting protein-protein interactions, including peptide design and viral fusion mechanisms.

3 Publications
Methodological Innovation

Development of standardized protocols, formulation studies, and comprehensive reviews for peptide-based drug discovery and therapeutic development.

2 Publications

Interested in Collaboration?

Open to discussing research opportunities, joint publications, and collaborative projects in peptide therapeutics and drug design.